Vocatıonal School
Medıcal Laboratory Technıques
Course Information
| MOLECULAR BIOLOGICAL TECHNIQUES | |||||
|---|---|---|---|---|---|
| Code | Semester | Theoretical | Practice | National Credit | ECTS Credit |
| Hour / Week | |||||
| TLT203 | Fall | 2 | 2 | 3 | 4 |
| Prerequisites and co-requisites | None |
|---|---|
| Language of instruction | Turkish |
| Type | Required |
| Level of Course | Associate |
| Lecturer | Lec. Tiinçe AKSAK |
| Mode of Delivery | Face to Face |
| Suggested Subject | None |
| Professional practise ( internship ) | None |
| Objectives of the Course | To provide students with knowledge and skills about molecular biological methods |
| Contents of the Course | General information about molecular methods, Molecular methods in laboratory, DNA isolation methods, PCR methods, Preparing PCR mix, Preparing agarose, Agarose gel electrophoresis, RFLP methods, Multiplex PCR method, Blotting techniques and usage. |
Learning Outcomes of Course
| # | Learning Outcomes |
|---|---|
| 1 | It isolates DNA from various body fluids and tissues. |
| 2 | Prepare agarose gel |
| 3 | Apply and evaluate molecular methods such as PCR |
| 4 | Making and evaluating RFLP |
| 5 | To learn and evaluate blotting techniques and their usage |
Course Syllabus
| # | Subjects | Teaching Methods and Technics |
|---|---|---|
| 1 | General information about molecular methods | Lecture, Powerpoint presentation, Question-answer, Practical method |
| 2 | The place and importance of molecular methods in routine laboratories | Lecture, Powerpoint presentation, Question-answer, Practical method |
| 3 | DNA isolation methods from microorganism, human blood and tissue samples | Lecture, Powerpoint presentation, Question-answer, Practical method |
| 4 | DNA isolation from microorganism, human blood and tissue samples | Lecture, Powerpoint presentation, Question-answer, Practical method |
| 5 | Polymerase chain reaction (PCR), real time PCR and PCR variants | Lecture, Powerpoint presentation, Question-answer, Practical method |
| 6 | Chemical substances and buffers required for PCR | Lecture, Powerpoint presentation, Question-answer, Practical method |
| 7 | To prepare PCR, to make Mix distribution and to provide amplification | Lecture, Powerpoint presentation, Question-answer, Practical method |
| 8 | Mid-Term Exam | Questions and Answers |
| 9 | Weighing and resolving the agarose, Preparing the agarose tank and pouring the agarose | Lecture, Powerpoint presentation, Question-answer, Practical method |
| 10 | Apply, execute and visualize agarose jeline DNA | Lecture, Powerpoint presentation, Question-answer, Practical method |
| 11 | Preparing the RFLP mixture, distributing the mixture and initiating the cut | Lecture, Powerpoint presentation, Question-answer, Practical method |
| 12 | To apply and evaluate the agarose jeline RFLP product | Lecture, Powerpoint presentation, Question-answer, Practical method |
| 13 | Prepare the multiplex PCR mix, make the distribution of the multiplex PCR mixture and start the amplification | Lecture, Powerpoint presentation, Question-answer, Practical method |
| 14 | Blotting Techniques | Lecture, Powerpoint presentation, Question-answer, Practical method |
| 15 | Blotting Techniques | Lecture, Powerpoint presentation, Question-answer, Practical method |
| 16 | Final Exam | Questions and Answers |
Course Syllabus
| # | Material / Resources | Information About Resources | Reference / Recommended Resources |
|---|---|---|---|
| 1 | Responsible Instructor Course Contents | ||
| 2 | Temizkan G, Arda N (2008). Moleküler Biyolojide Kullanılan Yöntemler, Nobel Tıp Kitabevi. | ||
| 3 | Muhsin Konuk (2004). Moleküler Biyoloji, Nobel yayınevi. | ||
| 4 | Hames BD, Rickwood D. (1990). Gel electrophoresis of proteins, Oxford Un. Pres. |
Method of Assessment
| # | Weight | Work Type | Work Title |
|---|---|---|---|
| 1 | 30% | Mid-Term Exam | Mid-Term Exam |
| 2 | 20% | Laboratory | Laboratory |
| 3 | 50% | Final Exam | Final Exam |
Relationship between Learning Outcomes of Course and Program Outcomes
| # | Learning Outcomes | Program Outcomes | Method of Assessment |
|---|---|---|---|
| 1 | It isolates DNA from various body fluids and tissues. | 1͵2͵3͵5͵6͵7͵8͵9͵10͵12͵13 | 1͵2͵3 |
| 2 | Prepare agarose gel | 1͵2͵3͵5͵6͵7͵8͵9͵10͵12͵13 | 1͵2͵3 |
| 3 | Apply and evaluate molecular methods such as PCR | 1͵2͵3͵5͵6͵7͵8͵9͵10͵12͵13 | 1͵2͵3 |
| 4 | Making and evaluating RFLP | 1͵2͵3͵5͵6͵7͵8͵9͵10͵12͵13 | 1͵2͵3 |
| 5 | To learn and evaluate blotting techniques and their usage | 1͵2͵3͵5͵6͵7͵8͵9͵10͵12͵13 | 1͵2͵3 |
Work Load Details
| # | Type of Work | Quantity | Time (Hour) | Work Load |
|---|---|---|---|---|
| 1 | Course Duration | 14 | 4 | 56 |
| 2 | Course Duration Except Class (Preliminary Study, Enhancement) | 14 | 4 | 56 |
| 3 | Presentation and Seminar Preparation | 0 | 0 | 0 |
| 4 | Web Research, Library and Archival Work | 0 | 0 | 0 |
| 5 | Document/Information Listing | 0 | 0 | 0 |
| 6 | Workshop | 0 | 0 | 0 |
| 7 | Preparation for Midterm Exam | 1 | 2 | 2 |
| 8 | Midterm Exam | 1 | 1 | 1 |
| 9 | Quiz | 0 | 0 | 0 |
| 10 | Homework | 0 | 0 | 0 |
| 11 | Midterm Project | 0 | 0 | 0 |
| 12 | Midterm Exercise | 1 | 2 | 2 |
| 13 | Final Project | 0 | 0 | 0 |
| 14 | Final Exercise | 0 | 0 | 0 |
| 15 | Preparation for Final Exam | 1 | 2 | 2 |
| 16 | Final Exam | 1 | 1 | 1 |
| 120 | ||||